KrosFlo^® KR2i RPM^™ System

Tangential Flow Filtration System with In-Line Protein Concentration Management

Finally, a Tangential Flow Filtration (TFF) system that monitors concentration during your UF/DF run. No longer are you dependent solely on mass inputs and off-line fixed-pathlength UV-Vis spectrophotometers. Now, you can measure what truly matters in real-time: your concentration.
With the power of the KrosFlo® KR2i Automated TFF System and the insights from the CTech™ FlowVPX® System, you can take control of your process.

Strengthen your process control with high accuracy and reproducible results.
Increase process efficiency by reducing cycling time and increasing yield throughput.
Reduce process risk by ensuring accurate concentration throughout the TFF process.

Power of Automation

Walk-away automation — 13 automated process control modes and manual operation, to meet your PD needs.

Minimize risk with fully enclosed ProConnex® custom flow paths, as part of the automated TFF process.

Robust processing allows for processing of volumes from 10 ml–10 L to meet both lab and clinical production requirements. Enables low volume, high concentration applications.

Flexible configuration — Compatible with flat sheet cassettes and hollow fiber filters with plug-n-play accessories for ultimate flexibility. Customizable flow path and small footprint.

Real-time Concentration Insights

In-Line integration of the FlowVPX instrument to UF/DF operations provides real-time protein concentration monitoring while allowing for feedback control.

Rapid Data Acquisition

generates 12 “QC-grade” concentration measurements in 1 minute (5 pathlengths in 5 seconds) with continuous data acquisition during the UF/DF process.

Broad Dynamic Range

allows for concentration measurement from 0.1 mg/ml to 300 mg/ml to be tested without dilution

Better Accuracy

Variable Pathlength Technology (VPT) allows the system to adjust to the right pathlength range for accurate measurements regardless of the molecule.

Integrated Data Analysis

Real-Time Process Management Software (KrosFlo® RPM™ Software)

Integrated software combines the power of the KR2i automation with the real-time insight from the FlowVPX System to give you the data you need and the control you’ve desired.
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TFF automation control based on in-line concentration
Provides real-time overview at every step of the filtration process
Automatically graphs data and trends of critical inputs and outputs
Flexible plug-and-play technology for custom needs

Better Insights to Drive Better Outcomes

By leveraging in-line concentration data, you are not required to do upfront calculation or overcompensate for variability.

Providing you with key process in-sights allows you to better under-stand your process—reduce cycling time and minimizing batch risk.

Focus on the end goal by eliminating a common source of error seen with concentration factor (intermediate characteristic).

Better Control Regardless of Process Variables

With in-line concentration data as the primary control factor for the UF/DF run, the process was able to reach target final concentration regardless of starting volume, crossflow rate, TMP, or retentate hold-up volume.

One Platform for Maximum Alignment

Variable pathlength technology is delivered through the FlowVPX System in real-time during your process. The CTech™ SoloVPE® System is the perfect compliment to the RPM System, allowing at-line or off-line analysis that provides the same accurate analysis of your product and process. A single technology platform that drives alignment across various steps and functions.

Bioprocess Step	CTech™ FlowVPX® System in-line (g/l)	CTech™ SoloVPE® System off-line (g/l)
UF1/Diafiltration	47.7	47.6
Ultrafiltration 2	226	229

KrosFlo^® KR2i RPM^™ System

Tangential Flow Filtration System with In-Line Protein Concentration Management

Power of Automation