With its patented variable pathlength technology, the SoloVPE System unlocks the power of Slope Spectroscopy.
Moving beyond traditional fixed pathlength spectroscopy, the SoloVPE solution expands the UV-Vis technique to create a three-dimensional science.
Conceptually simple, but analytically empowering, the SoloVPE solution delivers rapid and accurate results to avoid costly dilution and decreases process errors.
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Unlike the single value dependence of legacy UV-Vis methods, the data-dense slope method characterizes samples by collecting multiple absorbance data points at several pathlengths to create a section curve (absorbance vs. pathlength plot).
The section curve is then analyzed in real time to verify linearity in compliance with the Beer-Lambert law. The linear region of the section curve is directly proportional to the concentration of the sample based upon the sample extinction coefficient.
The result?
The SoloVPE System reports concentration results in less than 60 seconds. Capable of making spectral and fixed-point measurements at wavelengths between 190 nm and 1100 nm at pathlengths between 5 microns and 15 millimeters, the SoloVPE solution is adaptable to a wide range of sample types and concentrations. The SoloVPE System method has been proven to be accurate, precise, and robust. Therefore, it can easily and successfully be validated in accordance with ICH Q2(R1) requirements.
The variable pathlength technology allows R2 of ≥0.999 proving the system-to-system consistency is robust.
The flexibility and robustness of the technology is unparalleled when compared to traditional UV-Vis techniques and equipment.
Measure highly concentrated samples directly without dilution.
Buffer/ background correction only required in special circumstances.
Direct measurements of highly concentrated samples saves time and consumables.
Concentration results in about one minute.
Method and sample vessel dependent ranging from <20 μl to 2 ml can be reclaimed.
Confidently work system-to-system with +/- 2% repeatability.
Slope Spectroscopy Methods: The first and only slope spectrometer, capable of Slope Spectroscopy-based measurements. Slope results based upon multiple data points instead of a single absorbance value.
Rapid Sample Characterization: Rapidly characterize samples at different wavelengths and pathlengths using a single method.
Education/Support: On-site installation and training included with system purchase.
Linear Range Finder Technology: The system automatically identifies the linear region of Section data sets to verify compliance with the Beer-Lambert law.
Specification Sheet: View Document
Dimensions: 10” [255 mm] (W) x 10” [255 mm] (D) x 15” [381 mm] (H)
Weight: 20 lbs. [9 Kg]
*Spectroscopic Engine: Agilent Cary 60 Spectrophotometer Fiber Optic
Integration: C Technologies, Inc. Dual-Use Fiber Optic Coupler
SoloVPE Power Requirements: SoloVPE unit contains no power supply (powered via Cary 60)
Cary 60 Power Requirements: 100-240 V AC, Frequency 47-63 Hz
Maximum Pathlength: 15.000 mm
Minimum Pathlength Step: 0.005 mm
Variable Pathlength Speed: >1.3 mm/sec
**Slope Repeatability: ± 2%
Sample Vessel Compatibility: Silica (Large, Small, Micro), Disposable Plastic (Small)
Fibrette Compatibility: OF0002 (Silica + Polyimide)
Sample Volume Required: Dependent on sample vessel used and method pathlength range.
Proximity to Cary 60: On top of or within 0.5 m
‡Measurement Pathlength Axis Orientation: Vertical
Required Computer Hardware Per the minimum requirements of the Agilent Cary WinUV Software Package C Tech Recommends:
*Photometric performance characteristics are based upon the Cary 60 spectrophotometer specifications and are applicable to the Cary 60 independent of the SoloVPE.
**Repeatability performance requires properly validated method and controlled homogeneous samples.
‡Samples that are not homogeneous, suspensions, improperly mixed, or not in solution could produce unexpected results. This should be assessed during method development.
Protein A280; Monoclonal Antibodies
Planova Integrity Testing
Quantitation of Nucleic Acids (DNA/RNA)
Operating System: Microsoft Windows 7 & Windows 10 compatible
Software Environment: Agilent Cary WinUV Software Suite Version 5.0 / 5.1 through (1042)
VPT Software Control: C Technologies, Inc. SoloVPE Software Version 3.1
Validation Companion (Optional): C Technologies, Inc. QuickVCA for use with the SoloVPE Validation
Security Companion (Optional): C Technologies, Inc. SecureVPE Software Version 3.1 (for GxP implementations)
Prior Versions: Prior software versions may be available on request. Contact Sales for more information.
Legacy Support: Support for legacy products can be secured but subject to limitations.
Support Services
Enjoy the benefit of year-round discounts and support. We offer one to three year service contracts for your SoloVPE System. Schedule a visit from a Repligen Analytics Representative to qualify your SoloVPE System.
Application Service
Supplementary to our standard support options, application services provide you with increased access to our highly knowledgeable and experienced professionals. We offer implementation, development, and educational guidance to maximize the benefits of your variable pathlength solutions and the Slope Spectroscopy® technique.
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Bridgewater, NJ 08807 US
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