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SoloVPE Application: Plasmid DNA Purity

Measure Plasmid Purity With Unsurpassed Accuracy

The purest assessment you can get

The emergence of different high absorbance biotherapeutics modalities, such as oligonucleotides, presents a challenge in using traditional spectrophotometric methods for concentration measurements. Slope Spectroscopy^® offers an excellent alternative to traditional spectrophotometric analysis for nucleic acid quantitation and purity assessment using A260/A280 ratio measurement.

The SoloVPE Solution

Accurately measures DNA and plasma purity undiluted.
Eliminates the need for baseline and scatter correction.
Allows users to get an accurate R² value by measuring the 260 nm and 280 nm wavelength simultaneously.
Manipulates Beer’s law, allowing the System to accurately vary the absorbance with the change in pathlength.
Simultaneous wavelength measurement enables automated calculation of absorbance ratio.
There is no sample handling or sample prep, just direct measurement, while the variable pathlength allows R² of ≥0.999.
The MultiQ feature allows users to measure 260 nm and 280 nm simultaneously.

Customer Use Cases

Scientists at Pfizer validated the determination of antisense oligonucleotides using the SoloVPE System.

Publications

Our most recent research

Determination of Plasmid DNA Purity Ratio in Human Gene Therapy Products Using Slope Spectroscopy

Webinars

There are no webinars available at this time. Feel free to explore our resource library to learn more.

Application Services

The passion for what we do is in the product and support services you receive

CTech is committed to our customers’ success. We design service offerings to supplement our standard support options and provide you with increased access to our highly knowledgeable and experienced professionals. We offer implementation, development, and educational guidance to maximize the benefits of your variable pathlength solutions and the Slope Spectroscopy^® technique.

Application Services

21 CFR Part 11 Guidance
Annex 11 Guidance
Validation Support
Qualification Service
Method Transfer Service

Development

Method Design & Development
Comparability
On-Site Support
Custom Standards
Process Auditing

Education

SOP Document Review
Advanced Slope Spectroscopy Training
Data Analysis
Maintenance & Troubleshooting

Contact a Bioanalytics Application Specialist

Your Partners in Method Development and Process Solutions.

Connect with our dedicated applications team. Our team can show you how to maximize the benefits of your variable pathlength solutions and the Slope Spectroscopy® technique. Let us help you expedite your SoloVPE Validation.

SoloVPE Application: Plasmid DNA Purity

Measure Plasmid Purity With Unsurpassed Accuracy

The purest assessment you can get

The SoloVPE Solution

Customer Use Cases

Publications

Our most recent research

Determination of Plasmid DNA Purity Ratio in Human Gene Therapy Products Using Slope Spectroscopy

Determination of Plasmid DNA Purity Ratio in Human Gene Therapy Products Using Slope Spectroscopy

Webinars

Application Services

The passion for what we do is in the product and support services you receive

Application Services

Development

Education

Contact a Bioanalytics Application Specialist

Your Partners in Method Development and Process Solutions.

Our Products

Applications

Support

Connect