Application of Inline Variable Pathlength Chromatography for High Concentration Oligonucleotide Solutions

With the increased focus on oligonucleotides within biopharmaceutical projects, real time measurements of product concentration can lead to increased efficiency within bioprocessing and manufacturing. A collaboration between Repligen and Ionis Pharmaceuticals assessed the viability of in-line measurement of oligonucleotide concentration during reversed-phase chromatography. The experiment supports the implementation of in-line variable pathlength chromatography as a viable alternative for in-line variable pathlength spectrophotometer real-time integration of high concentration oligonucleotide solutions.