Determination of Plasmid DNA Purity Ratio in Human Gene Therapy Products Using Slope Spectroscopy

Estimation of DNA purity in the presence of protein for gene therapy plasmid material is possible by exploiting the 260/280 UV absorbance ratio. This technique, however, has significant challenges when accurate quantification of DNA purity is required. The SoloVPE and its Slope Spectroscopy technique offer a new method to overcome these challenges, and to accurately measure DNA purity ratios in accordance with the Beer-Lambert law.

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